Molecular epidemiology of outbreak of respiratory syncytial virus within bone marrow transplantation unit.

Molecular epidemiology of two consecutive outbreaks of parainfluenza 3 in a bone marrow transplant unit.

Two consecutive nosocomial outbreaks of parainfluenza 3, in which 5 of 15 infected patients died, occurred in an adult bone marrow transplant unit. Parainfluenza 3 strain variation was assessed by reverse transcription-PCR sequencing of part of the parainfluenza 3 F gene, including the noncoding region, directly from clinical samples. Sequence data from the outbreaks were compared with those fr...

Pulmonary Complications of Bone Marrow

BMT=bone marrow transplantation; BO=bronchiolitis obliterans; CMV=cytomegalovirus; DAH=diffuse alveo¬ lar hemorrhage; DCO=diffusing capacity ofcarbon mon¬ oxide; GVHD=graft-vs-host disease; HHV-6=human her¬ pes virus-6; HSV=herpes simplex virus; IPS=idiopathic pneumonia syndrome; PAP=pulmonary alveolar protei¬ nosis; FCF=Pneumocysti8 carinii pneumonia; PFT=pulmonary function test; RSV=respirato...

RNA fingerprinting of respiratory syncytial virus using ribonuclease protection. Application to molecular epidemiology.

We have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup A respiratory syncytial (RS) virus. RNAs extracted from HEp-2 cells infected with strains to be analyzed were hybridized with a 32P-labeled RNA probe corresponding to the RS virus G glycoprotein (A2 strain). Areas of nonhomology were detected by cleavage with ribonuclease A. ...

Pneumoviruses: Molecular Genetics and Reverse Genetics

Pneumoviruses are responsible for significant respiratory disease in their hosts and represent a major problemfor human and animal health. Pneumoviruses are members of the family Paramyxoviridae, subfamilyPneumovirinae and the virus particles consist of a negative-sense, nonsegmented RNA genome within a helical nucleocapsid structure enveloped in a lipid membrane derived from the ho...

Molecular Epidemiological Investigation of Human Parainfluenza 3 Virus Outbreak in a Pediatric Bone Marrow Transplantation Unit